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Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation

Paper ID Volume ID Publish Year Pages File Format Full-Text
5058 279 2006 7 PDF Available
Title
Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation
Abstract

Spodoptera frugiperda (Sf21) insect cells were grown in the microspheres that were prepared using sodium-cellulosesulfate (NaCS) and poly-diallyldimethylammoniumchloride (PDADMAC). The highest Sf21 cell density was 1 × 108 cells/ml in the microshperes. The immobilised Sf21 cells were infected with Autograpa californica multiple nuclear polyhedrosis virus (AcMNPV) at a “theoretical” MOI of 1.0 and a TOI of 3.9 × 107 cells/ml in the microspheres. The AcMNPV polyhedral inclusion bodies (PIBs) titer reached was 1.76 × 1010 PIBs/ml in the microspheres, which was 58 times higher than that (2.75 × 107 PIBs/ml) in a suspension condition. The immobilised Sf21 cells were infected with a recombinant baculovirus, Ac-omega-GFP baculovirus, and the highest concentration of green fluorescent protein (GFP) produced was 0.0048 mg/ml in suspension culture and 0.159 mg/ml in the microspheres.

Keywords
Immobilisation; NaCS; PDADMAC; Ac-omega-GFP baculovirus; PIBs; GFP
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Production of baculoviruses and expression of green fluorescent protein in immobilised Sf21 insect cell cultivation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 55–61
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us