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Effect of alkylamine on activity and stability of immobilized angiotensin converting enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
50593 46804 2012 4 PDF Available
Title
Effect of alkylamine on activity and stability of immobilized angiotensin converting enzyme
Abstract

Angiotensin converting enzyme (ACE) was immobilized on chitosan microspheres with glutaraldehyde as cross-linking reagent. The activity and stability of immobilized ACE (I-ACE) were improved by further modification with alkylamines. The modification conditions were optimized. The characteristics of I-ACE and modified I-ACE (MI-ACE) including activity, kinetic parameters, optimal pH and temperature, stability and reusability were investigated. After modification, I-ACE activity was increased, Km decreased from 4.33 mM to 2.61 mM, optimal pH value was altered, pH stability, storage stability and thermal stability were improved, and optimal temperature did not change.

Graphical abstractEffect of concentrations of methylamine, n-butylamine and ethanolamine on the activity of immobilized ACE. (The solutions were prepared with water, pH 8.3, adjusted by HCl.)The activity of immobilized ACE (I-ACE) could be improved significantly after modification. Methylamine, n-butylamine and ethanolamine exhibited activation in a broad concentration range. After modifying under the optimum condition (2240 mM methylamine), the activity of the modified I-ACE was 2 folds higher than that of I-ACE.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► ACE was immobilized on chitosan microspheres with glutaraldehyde. ► The modification aiming at quenching excess aldehyde groups in immobilized ACE. ► The activity and stability of immobilized ACE were improved by modification. ► The modification can be used to recover I-ACE activity after storage.

Keywords
Angiotensin converting enzyme; Modification; Alkylamine; Activity; Stability
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Effect of alkylamine on activity and stability of immobilized angiotensin converting enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 24, 5 July 2012, Pages 16–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us