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Fabrication and characterization of a PDMS–glass hybrid continuous-flow PCR chip

Paper ID Volume ID Publish Year Pages File Format Full-Text
5063 279 2006 7 PDF Available
Title
Fabrication and characterization of a PDMS–glass hybrid continuous-flow PCR chip
Abstract

A continuous-flow (CF) microchip was fabricated using polydimethylsiloxane (PDMS)/glass laminate for efficient polymerase chain reaction (PCR). The temperatures of the three separate zones, corresponding to the three PCR thermal cycling steps were controlled using an external PID temperature controller. A PCR mixture was introduced to the CF microchip using a syringe pump. The hydrophobic surface of the PDMS channel which adsorbs enzymes was modified by adding 2.5% polyvinylpyrrolidone (PVP) to the PCR mixture. The effect of flow rate in the CF chip on DNA amplification was examined and its PCR efficiency was compared with that of conventional PCR. The microchip requires only 8–30 min to produce detectable amounts of PCR products as determined by slab gel separation, whereas a conventional thermocycler requires 90–150 min over 30 amplification cycles to achieve the same result.

Keywords
Adsorption; DNA; Bioreactions; Heat transfer; Continuous-flow (CF) PCR; Polyvinylpyrrolidone (PVP)
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Fabrication and characterization of a PDMS–glass hybrid continuous-flow PCR chip
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 91–97
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us