fulltext.study @t Gmail

Colony lift immunoassay utilizing antibody-coupled liposomes encapsulating HRP

Paper ID Volume ID Publish Year Pages File Format Full-Text
5064 279 2006 5 PDF Available
Title
Colony lift immunoassay utilizing antibody-coupled liposomes encapsulating HRP
Abstract

The liposome immunoblotting assay was applied to colony lift immunoassay for detection of colonies expressing target protein (scFv) with higher signal intensity. Only edges of blotted samples on the membrane were developed by a direct blotting method, because cells adsorbed on the surface of the membrane plugged openings of pores and physically prevented interaction between the antigen and immunoliposomes. By an indirect blotting method, uniform signals with higher density were successfully observed from blotted membranes by use of immunoliposomes. Immunoliposome of large unilamellar vesicles (immuno-LUVs) showed the highest S/N ratio among three kinds of liposomes. In the colony lift imunoassay utilizing immuno-LUVs, the signal density was three times higher, compared with the conventional assay, while their background levels were almost same. Consequently, a higher S/N ratio could be attained for detection of colonies secreting active scFv. Thus, this method is useful to screen colonies expressing target protein with higher sensitivity.

Keywords
Immunoliposomes; Blotting assay; Colony lift immunoassay
First Page Preview
Colony lift immunoassay utilizing antibody-coupled liposomes encapsulating HRP
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 98–102
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us