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Detection of Escherichia coli in the sewage influent by fluorescent labeled T4 phage

Paper ID Volume ID Publish Year Pages File Format Full-Text
5067 279 2006 6 PDF Available
Title
Detection of Escherichia coli in the sewage influent by fluorescent labeled T4 phage
Abstract

For a precise estimation of sanitary condition, Escherichia coli detection system using E. coli-specific bacteriophage T4 was constructed. To facilitate E. coli detection, T4e− phage which did not produce the lysozyme was constructed and green fluorescent protein (GFP) was displayed on T4e− small outer capside (SOC) protein. This T4e−/GFP can detect E. coli K12 without cell lysis. In this study, we applied T4e−/GFP to the detection of E. coli in the sewage influent.Chromocult® coliform (CC) agar plates are generally used for simultaneous detection of total coliforms and E. coli. We investigated the number of coliforms and E. coli in the municipal sewage influent by using CC agar plates for 1 year. There were 105–106 CFU/ml of total coliforms and 104–105 CFU/ml of E. coli throughout the year. More than 20 strains of E. coli selected from CC agar were infected by T4e−/GFP. The ratio of clear plaque forming E. coli was different every month and very low (annual average 8%). Most of the E. coli showed turbid plaque or no plaque. E. coli formed the turbid plaque showed no-fluorescence, fluorescence only on cell surface due to phage adsorption, or heterogeneous fluorescence among the cells, while E. coli formed clear plaque could be detected because of T4e−/GFP amplification in the cell.

Keywords
Escherichia coli; Fluorescent labeled phage; Sewage influent; Chromocult® coliform agar
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Detection of Escherichia coli in the sewage influent by fluorescent labeled T4 phage
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 119–124
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us