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A kinetic study on the aggregation behavior of β-amyloid peptides in different initial solvent environments

Paper ID Volume ID Publish Year Pages File Format Full-Text
5069 279 2006 10 PDF Available
Title
A kinetic study on the aggregation behavior of β-amyloid peptides in different initial solvent environments
Abstract

β-Amyloid peptide (Aβ) is the major proteinacious constituent of senile plaques in Alzheimer's disease and is believed to be responsible for the neurodegeneration associated with the disease. This work is aimed at determining the effect of solvent environment on the aggregation kinetics of Aβ peptides. Prior to dilution into phosphate buffer saline, we have used three different initial solvent systems, 0.1% (v/v) trifluoroacetic acid in deionized water, 100% (v/v) dimethylsulfoxide, and 8 M urea to solubilize Aβ peptides. Our research shows that the increase in ThT fluorescence intensity or absorbance elicited by aggregated species of Aβ peptides exhibited a solvent environment-dependent behavior. Results from Aβ aggregation in trifluoroacetic acid–phosphate buffer saline and dimethylsulfoxide–phosphate buffer saline systems suggested the involvement of the seeding effect. Moreover, with the aid of three proposed reaction schemes, the effect of this solvent environment-dependent behavior was quantitatively analyzed. We believe that a better understanding of how Aβ species and its derivatives aggregate/self-assemble will shed light on the design and analysis of future work in this area.

Keywords
Alzheimer's disease; β-Amyloid; Aggregation; Kinetics; Solvent
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A kinetic study on the aggregation behavior of β-amyloid peptides in different initial solvent environments
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 29, Issues 1–2, 1 April 2006, Pages 129–138
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us