fulltext.study @t Gmail

Catalytic potential of cauliflower (Brassica oleracea) bud peroxidase in decolorization of synthetic recalcitrant dyes using redox mediator

Paper ID Volume ID Publish Year Pages File Format Full-Text
50757 46812 2011 6 PDF Available
Title
Catalytic potential of cauliflower (Brassica oleracea) bud peroxidase in decolorization of synthetic recalcitrant dyes using redox mediator
Abstract

We investigated the effect of cauliflower (Brassica oleracea) bud peroxidase along with redox mediators on decolorization of dyes (Reactive Red 2, Reactive Black 5, Reactive Blue 4, Disperse Orange 25 and Disperse Black 9). Results indicated that among the chosen mediators, 1-hydroxybenzotriazole (HOBT) followed by riboflavin were most effective for dye decolorization. At 0.36 EU/ml and with 0.8 mM HOBT in conjunction with 0.75 mM H2O2 the soluble cauliflower bud peroxidase proteins could decolorize the dyes. The enzyme worked in a broader range of pH and temperature and was adequately effective in batch processes when used with organic contaminants (dioxane and dimethylformide), detergents (Triton X-100 and sodium dodecyl sulfate), sodium chloride and heavy metals including zinc and cadmium chloride. Thus peroxidase from cauliflower bud is a better choice than other vegetable peroxidases as it is sufficiently thermostable, operates in a wide range of pH, economic and effective with low concentration of redox mediators in decolorizing recalcitrant synthetic dyes. Such peroxidases with better catalytic activity have potential of being used on a large scale.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Cauliflower bud peroxidase tested for dye color removal in batch process. ► Optimum activity with 0.8 mM HOBT/0.36 EU SCBP/ml/0.75 mM H2O2/pH (3–7). ► Performance examined in presence of Triton X-100/SDS/NaCl/ZnCl2/CdCl2. ► Turns out to be potent and more effective than other tested vegetable peroxidases.

Keywords
Synthetic dyes; Cauliflower bud peroxidase; Decolorization; Redox mediator; Dioxane and dimethylformide; Detergents
First Page Preview
Catalytic potential of cauliflower (Brassica oleracea) bud peroxidase in decolorization of synthetic recalcitrant dyes using redox mediator
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 15, Issue 1, 15 November 2011, Pages 93–98
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us