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Optimization of lipase production in a triple impeller bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
5086 283 2006 8 PDF Available
Title
Optimization of lipase production in a triple impeller bioreactor
Abstract

The fermentation kinetics for the synthesis of lipase by Candida rugosa has been studied in a batch system in a 2 l batch bioreactor. The studies illustrated the influence of gas–liquid mass transfer coefficient on the cell growth and hence the lipase production. In order to maintain sufficient oxygen concentration for the optimum cell growth and lipase activity, fermentation has been carried out using triple impeller system at an operating speed of 600 rpm (optimum operating speed as found in the earlier work) and at different aeration rates. Gas-flow rate of 50.34 cc/s has been observed to be optimum. Under optimized conditions of the bioreactor, cell production was enhanced and the lipase activity increased by 2.5 folds. The Monod's kinetics was fitted to the data of the operating parameters to understand the cell growth and substrate consumption. Luedking and Piret model was applied to the data to determine the relationship between the cell growth and lipase production. The lipase production was found to be microbial growth associated function.

Keywords
Bioreactor; Gas–liquid mass transfer; Fermentation; Triple impeller; Aerobic process; Growth kinetics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 27, Issue 3, January 2006, Pages 287–294
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us