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Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine

Paper ID Volume ID Publish Year Pages File Format Full-Text
50869 46819 2010 4 PDF Available
Title
Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine
Abstract

Polyethyleneimine (PEI) modification on immobilized lipases greatly enhanced their enantioselectivity in the kinetic resolution of (±)-2-hydroxy-phenylacetic acid methyl ester. The enantiomeric ratio (E) of CNBr-agarose-CRL rose from 8 (without coating) to 20 (ee = 90%) after PEI coating in the hydrolysis at pH 5. In the case of CAL-B, the coating highly improved the enantioselectivity of the immobilized lipase from E = 1.5 (without coating) to E > 100 (ee > 99%). Moreover, this coating strategy improved the stability of the biocatalyst at high temperatures and in the presence of high co-solvent concentrations.

Keywords
Protein modification; Polymers; Enantioselectivity; Candida antarctica lipase; Candida rugosa lipase
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Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 11, Issue 11, 10 June 2010, Pages 964–967
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us