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Catalytic characteristics of CYP3A22-dependent mequindox detoxification

Paper ID Volume ID Publish Year Pages File Format Full-Text
51113 46830 2011 7 PDF Available
Title
Catalytic characteristics of CYP3A22-dependent mequindox detoxification
Abstract

The anti-bacterial agent mequindox is widely used as an animal feed additive in China. Its potential effect on host cells, however, remains largely unknown. Here, we report that pig CYP3A22 represents an important metabolic enzyme in mequindox catalysis. Interestingly, we found that ectopic expression of CYP3A22 reversed the mequindox-induced G2 cell cycle accumulation in cultured pig hepatocytes. To further probe the catalytic mechanism of CYP3A22 in mequindox metabolism, we identified 2′-acetyl-hydroxylation as a major catalytic step of CYP3A22-dependent mequindox metabolism using LC–MS/MS spectrometry. Together, our study suggests that CYP3A22 plays an important role in mequindox metabolism via 2′-acetyl-hydroxylation.

Graphical AbstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch Highlights►Mequindox induced G2 arrest of pig hepatocytes. ►High expression of CYP3A22 reversed the drug-induced G2 arrest. ►The catalytic activity of CYP3A22 is crucial for the mequindox detoxification.

Keywords
Mequindox; CYP3A22; Hydroxylation; Carbonyl reduction
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Catalytic characteristics of CYP3A22-dependent mequindox detoxification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 12, Issue 7, 10 March 2011, Pages 637–643
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us