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Rapid capture and exemplary detection of clinical pathogen using surface modified fluorescent silica coated iron oxide nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
5172 346 2014 8 PDF Available
Title
Rapid capture and exemplary detection of clinical pathogen using surface modified fluorescent silica coated iron oxide nanoparticles
Abstract

Rapid, sensitive and selective detection of pathogenic bacteria is very important for treatment of diseases like foodborne illness, sepsis and bioterrorism. The silica coated iron oxide nanoparticles (SIO) were synthesized using simple, cost-effective method and used for the rapid capture and detection of clinical pathogen. The surface modification of nanoparticles was carried out using 3-aminopropyltriethoxy silane. The scanning electron microscopy image results showed the slightly agglomerated spherical shaped nanoparticles. Transmission electron microscope result showed the polydispersed particles in the size ranges from 5 to 12 nm. The EDAX results confirmed the coating of silica with iron oxide particles. The SAED pattern confirmed the crystalline nature of iron oxide nanoparticles and also indicated the presence of silica. The FTIR spectrum of the nanoparticles confirmed the functional groups of the iron oxide and surface modified fluorescent silica coated iron oxide nanoparticles (SFSIO). This work provides a very effective method for controlling the growth, capture and detection of pathogenic bacteria.

Keywords
Bio-imaging; FITC; E. coli; Fluorescence microscope; Scanning electron microscope; Transmission electron microscope
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Rapid capture and exemplary detection of clinical pathogen using surface modified fluorescent silica coated iron oxide nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biocybernetics and Biomedical Engineering - Volume 34, Issue 4, 2014, Pages 230–237
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us