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Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids

Paper ID Volume ID Publish Year Pages File Format Full-Text
51722 46851 2011 7 PDF Available
Title
Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids
Abstract

This study deals with the preparation of surface-modified mesoporous activated carbon (SMMAC) using ethylenediamine and glutaraldehyde. Surface modification renders the covalent immobilization of acidic lipase (AL) onto MAC. The AL was produced from Pseudomonas gessardii using goat tallow as the substrate and purified. The maximum immobilization capacity of SMMAC for AL was 5440 U/g. The SMMAC and ALSMMAC were characterized for physico-chemical, morphological and material structure. The ALSMMAC showed credible hydrolytic efficiency toward slaughterhouse lipid wastes and vegetable oils. The hydrolysis of slaughterhouse lipid wastes and vegetable oils obeyed pseudo first order kinetic model. The excellent operational stability of ALSMMAC was verified using olive oil.

Graphical abstractSchematic illustration of the surface modification of MAC and covalent immobilization of AL onto SMMAC. SEM images of SMMAC2 and ALSMMAC2.Figure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Our study provides surface modified mesoporous activated carbon catalyst. ► Surface modification creates multipoint covalent immobilization of acidic lipase. ► SEM, TGA and FTIR studies confirmed surface modification and covalent immobilization. ► SMMAC had higher immobilization capacity than MAC. ► ALSMMAC showed better operational stability than the ALMAC.

Keywords
Surface-modified mesoporous activated carbon; Acidic lipase; Covalent immobilization; Biocatalyst; Lipid hydrolysis
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Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 14, Issue 1, 25 October 2011, Pages 82–88
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us