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Characterization and application of a sterol esterase immobilized on polyacrylate epoxy-activated carriers (Dilbeads™)

Paper ID Volume ID Publish Year Pages File Format Full-Text
52048 46863 2008 7 PDF Available
Title
Characterization and application of a sterol esterase immobilized on polyacrylate epoxy-activated carriers (Dilbeads™)
Abstract

The sterol esterase from the ascomycete Ophiostoma piceae was immobilized on novel polyacrylate-based epoxy-activated carriers (Dilbeads™). Six supports with particle sizes between 120 and 165 μm were prepared varying the composition of monomers, cross-linkers and porogens. Their surface areas and porosities were determined by N2 adsorption and mercury intrusion porosimetry. The pore volumes ranged from 0.63 to 1.32 cm3/g, but only Dilbeads™ RS and NK had narrow pore size distributions (with maxima at 33.5 and 67.0 nm, respectively). The distribution of the enzyme in the support was studied by fluorescence confocal microscopy. The immobilized esterase on Dilbeads™ TA showed a significant pH and thermal stability and was assayed in the continuous hydrolysis of cholesteryl esters –present in the pulp industry process waters.

Keywords
Covalent immobilization; Epoxy carriers; Cholesterol esterase; Confocal microscopy; Pitch control
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Characterization and application of a sterol esterase immobilized on polyacrylate epoxy-activated carriers (Dilbeads™)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Communications - Volume 9, Issue 4, 15 March 2008, Pages 539–545
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us