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Painting factor H onto mesenchymal stem cells protects the cells from complement- and neutrophil-mediated damage

Paper ID Volume ID Publish Year Pages File Format Full-Text
5315 366 2016 11 PDF Available
Title
Painting factor H onto mesenchymal stem cells protects the cells from complement- and neutrophil-mediated damage
Abstract

Mesenchymal stem cells (MSCs) are undergoing intensive testing in clinical trials as a promising new therapy for many inflammatory diseases and for regenerative medicine, but further optimization of current MSC-based therapies is required. In this study, we found that in addition to direct complement–mediated attack through the assembly of membrane attack complexes (MACs) that we and others have recently reported, of the released complement activation products, C5a, but not C3a, activates neutrophils in the blood to further damage MSCs through oxidative burst. In addition, we have developed a simple method for painting factor H, a native complement inhibitor, onto MSCs to locally inhibit complement activation on MSCs. MSCs painted with factor H are protected from both MAC- and neutrophil-mediated attack and are significantly more effective in inhibiting antigen-specific T cell responses than the mock-painted MSCs both in vitro and in vivo.

Keywords
MSC; Complement; Neutrophil; Therapy; Factor H
First Page Preview
Painting factor H onto mesenchymal stem cells protects the cells from complement- and neutrophil-mediated damage
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 102, September 2016, Pages 209–219
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering