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Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine

Paper ID Volume ID Publish Year Pages File Format Full-Text
53562 46974 2016 8 PDF Available
Title
Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine
Abstract

•The optimization of BpNDT immobilization is here reported.•Crosslinking of enzyme adsorbed to functionalized supports increased its stability.•Addition of 20% glycerol to the immobilization medium improved final activity.•Selected BpNDT biocatalyst was stable in presence of 20% DMF or high pH.•Immobilized BpNDT was used to synthesize decytabine and trifluridine.

The immobilization of Bacillus psychrosaccharolyticus nucleoside 2′-deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis of trifluridine and decytabine obtaining conversions ranging from 50% to 76%.

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Keywords
Nucleoside 2′-deoxyribosyltransferase; Biotransformations; Enzyme immobilization; Nucleoside analogues; Decytabine; Trifluridine
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Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Today - Volume 259, Part 1, 1 January 2016, Pages 197–204
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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