fulltext.study @t Gmail

Synthesis of cetyl ricinoleate catalyzed by immobilized Lipozyme® CalB lipase in a solvent-free system

Paper ID Volume ID Publish Year Pages File Format Full-Text
53766 46983 2015 5 PDF Available
Title
Synthesis of cetyl ricinoleate catalyzed by immobilized Lipozyme® CalB lipase in a solvent-free system
Abstract

•An immobilized CalB lipase with protein content over 30 mg/g has been obtained.•This immobilized CalB lipase was used as biocatalyst to produce cetyl ricinoleate.•The highest reaction rate value was obtained at 70 °C.•The vacuum-obtained cetyl ricinoleate reached an acid value of 1.98 mg KOH/g.•The derivative can be used three consecutive cycles without loss of activity.

A green process has been performed for the synthesis of the emollient ester cetyl ricinoleate with a new immobilized derivative of Candida antarctica lipase B, which has been prepared by physical adsorption of Lipozyme® CalB L on a macroporous anionic exchange resin (Lewatit® MonoPlus MP 64). An immobilized CalB lipase with protein content over 30 mg/g has been obtained and it has been successfully used as biocatalyst to produce cetyl ricinoleate from esterification of ricinoleic acid with cetyl alcohol. Influence of amount of biocatalyst and temperature was studied in the open-air reactor, and optimal values could be fixed in 2 mg and 70 °C, respectively. Biocatalyst storage stability study was developed in this reactor and it was showed the high storage stability of the immobilized derivative, because it keeps 100% of its enzymatic activity after eight months. Studies of recovery and reuse of the immobilized derivative were performed in the vacuum reactor, and it was proved the possibility of using the same biocatalyst in three consecutive reaction cycles without apparent loss of activity. Finally, the characterization of the cetyl ricinoleate obtained in the vacuum reactor demonstrated that the product obtained after only 3 or 4 h of reaction meets manufacturers’ specifications.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (192 K)Download as PowerPoint slide

Keywords
Candida antarctica lipase B; Enzymatic synthesis; Solvent-free; Cetyl ricinoleate; Cosmetics
First Page Preview
Synthesis of cetyl ricinoleate catalyzed by immobilized Lipozyme® CalB lipase in a solvent-free system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Today - Volume 255, 15 October 2015, Pages 49–53
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us