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Ultrasound-guided imaging of junctional adhesion molecule-A-targeted microbubbles identifies vulnerable plaque in rabbits

Paper ID Volume ID Publish Year Pages File Format Full-Text
5380 371 2016 11 PDF Available
Title
Ultrasound-guided imaging of junctional adhesion molecule-A-targeted microbubbles identifies vulnerable plaque in rabbits
Abstract

Identification of vulnerable atherosclerotic plaques by imaging the molecular characteristics is intensively studied recently, in which verification of specific markers is the critical step. JAM-A, a junctional membrane protein, is involved in the plaque formation, while it is unknown whether it can serve as a marker for vulnerable plaques. Vulnerable and stable plaques were created in rabbits with high cholesterol diet with or without partial ligation of carotid artery respectively. Significant higher JAM-A expression was found in vulnerable plaques than that in stable plaques. Furthermore, JAM-A was not only expressed in the endothelium, but also abundantly expressed in CD68-positive area. Next, JAM-A antibody conjugated microbubbles (MBJAM-A) or control IgG-conjugated microbubbles (MBC) were developed by conjugating the biotinylated antibodies to the streptavidin modified microbubbles, and visualization by contrast-enhance ultrasound (CEUS). Signal intensity of MBJAM-A was substantially enhanced and prolonged in the vulnerable plaque and some of the MBJAM-A was found colocalized with CD68 positive macrophages. In addition, cell model revealed that MBJAM-A were able to be phagocytized by activated macrophages. Taken together, we have found that increase of JAM-A serves as a marker for vulnerable plaques and targeted CEUS would be possibly a novel non-invasive molecular imaging method for plaque vulnerability.

Keywords
Atherosclerosis; Vulnerable plaque; JAM-A; Ultrasound contrast imaging; MacrophagesAS, atherosclerosis; CCA, common carotid artery; CEUS, contrast-enhance ultrasound; HFD, high fat diet; JAM-A, junctional adhesion molecule-A; MB, microbubble; α-SMA, smoot
First Page Preview
Ultrasound-guided imaging of junctional adhesion molecule-A-targeted microbubbles identifies vulnerable plaque in rabbits
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 94, July 2016, Pages 20–30
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering