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In vivo and in vitro evaluation of hydroxyapatite nanoparticle morphology on the acute inflammatory response

Paper ID Volume ID Publish Year Pages File Format Full-Text
5388 372 2016 11 PDF Available
Title
In vivo and in vitro evaluation of hydroxyapatite nanoparticle morphology on the acute inflammatory response
Abstract

Biomedical implants have been widely used in bone repair applications. However, nanosized degradation products from these implants could elicit an inflammatory reaction, which may lead to implant failure. It is well known that the size, chemistry, and charge of these nanoparticles can modulate this response, but little is known regarding the role that the particle's morphology plays in inducing inflammation. The present study aims to investigate the effect of hydroxyapatite nanoparticle (HANPs) morphology on inflammation, in-vitro and in-vivo. Four distinct HANP morphologies were fabricated and characterized: long rods, dots, sheets, and fibers. Primary human polymorphonuclear cells (PMNCs), mononuclear cells (MNCs), and human dermal fibroblasts (hDFs) were exposed to HANPs and alterations in cell viability, morphology, apoptotic activity, and reactive oxygen species (ROS) production were evaluated, in vitro. PMNCs and hDFs experienced a 2-fold decrease in viability following exposure to fibers, while MNC viability decreased 5-fold after treatment with the dots. Additionally, the fibers stimulated an elevated ROS response in both PMNCs and MNCs, and the largest apoptotic behavior for all cell types. Furthermore, exposure to fibers and dots resulted in greater capsule thickness when implanted subcutaneously in mice. Collectively, these results suggest that nanoparticle morphology can significantly impact the inflammatory response.

Keywords
Hydroxyapatite nanoparticles; Morphology; Mononuclear cells; Polymorphonuclear cells; Reactive oxygen species; In vivo
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In vivo and in vitro evaluation of hydroxyapatite nanoparticle morphology on the acute inflammatory response
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 90, June 2016, Pages 1–11
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us