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Hydroxychloroquine-conjugated gold nanoparticles for improved siRNA activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
5394 372 2016 10 PDF Available
Title
Hydroxychloroquine-conjugated gold nanoparticles for improved siRNA activity
Abstract

Current technology of siRNA delivery relies on pharmaceutical dosage forms to route maximal doses of siRNA to the tumor. However, this rationale does not address intracellular bottlenecks governing silencing activity. Here, we tested the impact of hydroxychloroquine conjugation on the intracellular fate and silencing activity of siRNA conjugated PEGylated gold nanoparticles. Addition of hydroxychloroquine improved endosomal escape and increased siRNA guide strand distribution to the RNA induced silencing complex (RISC), both crucial obstacles to the potency of siRNA. This modification significantly improved gene downregulation in cellulo. Altogether, our data suggest the benefit of this modification for the design of improved siRNA delivery systems.

Graphical abstractWe report hydroxychloroquine conjugation onto siRNA-functionalized PEGylated nanoparticles. Hydroxychloroquine modification enhanced endosomal escape, which improved RISC engagement of transfected siRNA and resulted in increased silencing activity over siRNA-functionalized PEGylated gold nanoparticles devoid of hydroxychloroquine.Figure optionsDownload full-size imageDownload high-quality image (213 K)Download as PowerPoint slide

Keywords
siRNA delivery; Hydroxychloroquine; Gold nanoparticles; Intracellular bioavailability
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Hydroxychloroquine-conjugated gold nanoparticles for improved siRNA activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 90, June 2016, Pages 62–71
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us