fulltext.study @t Gmail

Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
548 46 2013 9 PDF Available
Title
Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells
Abstract

The uptake of calcium phosphate nanoparticles (diameter 120 nm) with different charge by HeLa cells was studied by flow cytometry. The amount of uptaken nanoparticles increased with increasing concentration of nanoparticles in the cell culture medium. Several inhibitors of endocytosis and macropinocytosis were applied to elucidate the uptake mechanism of nanoparticles into HeLa cells: wortmannin, LY294002, nocodazole, chlorpromazine and nystatin. Wortmannin and LY294002 strongly reduced the uptake of anionic nanoparticles, which indicates macropinocytosis as uptake mechanism. For cationic nanoparticles, the uptake was reduced to a lesser extent, indicating a different uptake mechanism. The localization of nanoparticles inside the cells was investigated by conjugating them with the pH-sensitive dye SNARF-1. The nanoparticles were localized in lysosomes after 3 h of incubation.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (42 K)Download as PowerPoint slide

Keywords
Calcium phosphate; Nanoparticles; Endocytosis; Inhibitors
First Page Preview
Mechanism of the uptake of cationic and anionic calcium phosphate nanoparticles by cells
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 9, Issue 7, July 2013, Pages 7527–7535
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us