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Pure silica nanoparticles for liposome/lipase system encapsulation: Application in biodiesel production

Paper ID Volume ID Publish Year Pages File Format Full-Text
54902 47030 2013 8 PDF Available
Title
Pure silica nanoparticles for liposome/lipase system encapsulation: Application in biodiesel production
Abstract

In this work we report the synthesis of organic–inorganic solid with spherical morphology where enzyme, as active compounds, is encapsulated. The organic phase of nanospheres is composed of l-α-phosphatidylcholine, as liposome, and lipase from Rhizomucor miehei, as enzyme. The organic phase is covered with porous inorganic silica shell that could stabilize the internal liposomal phase and, consequently, isolate and protect the bioactive molecules. The liposome and silica amount used during the immobilization procedure have been optimized in order to obtain active and stable heterogeneous biocatalyst. Hybrid-nanospheres containing the enzyme were used to catalyze the transesterification reaction of triolein with methanol to methyl esters, typical biodiesel mixture compounds. The encapsulated enzyme retains its activity after 5 reaction cycles. The total productivity of the best catalyst obtained is higher than that of the free enzyme.

Graphical abstractSilica nanoparticles could be employed for storage of active biomolecules: their biocompatibility and stability toward external agents make them attractive systems to overcome the stability drawbacks of organic molecules. Enzyme-containing liposomes are interesting systems in which the biocatalyst is immobilized retaining its free and stable conformation, due to the biocompatible microenvironment inside the liposome membrane. Hybrid-nanospheres containing the lipase enzyme were used to catalyze the transesterification reaction of triolein with methanol to methyl esters, typical biodiesel mixture compounds. The encapsulated enzyme retains its activity after 5 reaction cycles. The total productivity of the best catalyst obtained is higher than that of the free enzyme.Figure optionsDownload full-size imageDownload high-quality image (124 K)Download as PowerPoint slideHighlights► Active biomolecules for biodiesel production have been synthesized. ► Liposomes hybrid-nanospheresis a biocompatible microenvironment for lipase enzyme. ► External porous silica shell stabilizes the bioactive molecule. ► Productivity of best heterogeneous biocatalyst is higher than that of the free enzyme.

Keywords
Lipase; Liposome; Hybrid-nanospheres; Biodiesel; Heterogeneous Catalysis
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Pure silica nanoparticles for liposome/lipase system encapsulation: Application in biodiesel production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Catalysis Today - Volume 204, 15 April 2013, Pages 148–155
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Catalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us