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Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
5500 394 2016 12 PDF Available
Title
Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration
Abstract

The use of artificial tissues in regenerative medicine is limited due to hypoxia. As a strategy to overcome this drawback, we have shown that photosynthetic biomaterials can produce and provide oxygen independently of blood perfusion by generating chimeric animal-plant tissues during dermal regeneration. In this work, we demonstrate the safety and efficacy of photosynthetic biomaterials in vivo after engraftment in a fully immunocompetent mouse skin defect model. Further, we show that it is also possible to genetically engineer such photosynthetic scaffolds to deliver other key molecules in addition to oxygen. As a proof-of-concept, biomaterials were loaded with gene modified microalgae expressing the angiogenic recombinant protein VEGF. Survival of the algae, growth factor delivery and regenerative potential were evaluated in vitro and in vivo. This work proposes the use of photosynthetic gene therapy in regenerative medicine and provides scientific evidence for the use of engineered microalgae as an alternative to deliver recombinant molecules for gene therapy.

Keywords
Regenerative medicine; Drug delivery; Molecular therapy; Biomaterials; Algae biotechnology; Oxygen; Hypoxia
First Page Preview
Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 75, January 2016, Pages 25–36
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering