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Efficient bridging of 20 mm rat sciatic nerve lesions with a longitudinally micro-structured collagen scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
5503 394 2016 11 PDF Available
Title
Efficient bridging of 20 mm rat sciatic nerve lesions with a longitudinally micro-structured collagen scaffold
Abstract

•Collagen-based multichannel scaffold is supportive of sciatic nerve axon regeneration.•Both sensory and motor axons regenerate through the scaffold.•Schwann cell seeding of scaffold does not improve its regenerative properties.•Collagen scaffold and autografts are equipotent in inducing functional recovery.

An increasing number of biomaterial nerve guides has been developed that await direct comparative testing with the ‘gold-standard’ autologous nerve graft in functional repair of peripheral nerve defects. In the present study, 20 mm rat sciatic nerve defects were bridged with either a collagen-based micro-structured nerve guide (Perimaix) or an autologous nerve graft. Axons regenerated well into the Perimaix scaffold and, the majority of these axons grew across the 20 mm defect into the distal nerve segment. In fact, both the total axon number and the number of retrogradely traced somatosensory and motor neurons extending their axons across the implant was similar between Perimaix and autologous nerve graft groups. Implantation of Schwann cell-seeded Perimaix scaffolds provided only a beneficial effect on myelination within the scaffold. Functional recovery supported by the implanted, non-seeded Perimaix scaffold was as good as that observed after the autologous nerve graft, despite the presence of thinner myelin sheaths in the Perimaix implanted nerves. These findings support the potential of the Perimaix collagen scaffold as a future off-the-shelf device for clinical applications in selected cases of traumatic peripheral nerve injury.

Keywords
Peripheral nerve injury; Nerve reconstruction; Neurotmesis; Epineurium; Perineurium; Autologous nerve transplantation; Cross-linking; Nerve guideAD, axon diameter; ADens, axonal density; ANT, autologous nerve transplantation; DCS, distal coaptation site;
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Efficient bridging of 20 mm rat sciatic nerve lesions with a longitudinally micro-structured collagen scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 75, January 2016, Pages 112–122
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us