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Selective characterization of proteins on nanoscale concave surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
5515 394 2016 8 PDF Available
Title
Selective characterization of proteins on nanoscale concave surfaces
Abstract

Nanoscale curvature plays a critical role in nanostructure-biomolecule interactions, yet the understanding of such effects in concave nanostructures is still very limited. Because concave nanostructures usually possess convex surface curvatures as well, it is challenging to selectively study the proteins on concave surfaces alone. In this work, we have developed a novel and facile method to address this issue by desorbing proteins on the external surfaces of hollow gold nanocages (AuNG), allowing the selective characterization of retained proteins immobilized on their internal concave surfaces. The selective desorption of proteins was achieved via varying the solution ionic strength, and was demonstrated by both calculation and experimental comparison with non-hollow nanoparticles. This method has created a new platform for the discrete observation of proteins adsorbed inside AuNG hollow cores, and this work suggests an expanded biomedical application space for hollow nanomaterials.

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Keywords
Gold nanoparticle; Concave nanostructure; Protein adsorption; Nano-bio interaction
First Page Preview
Selective characterization of proteins on nanoscale concave surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 75, January 2016, Pages 305–312
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering