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Impact of protein pre-coating on the protein corona composition and nanoparticle cellular uptake

Paper ID Volume ID Publish Year Pages File Format Full-Text
5521 394 2016 10 PDF Available
Title
Impact of protein pre-coating on the protein corona composition and nanoparticle cellular uptake
Abstract

Nanoparticles (NPs) are functionalized with targeting ligands to enable selectively delivering drugs to desired locations in the body. When these functionalized NPs enter the blood stream, plasma proteins bind to their surfaces, forming a protein corona that affects NP uptake and targeting efficiency. To address this problem, new strategies for directing the formation of a protein corona that has targeting capabilities are emerging. Here, we have investigated the feasibility of directing corona composition to promote targeted NP uptake by specific types of cells. We used the well-characterized process of opsonin-induced phagocytosis by macrophages as a simplified model of corona-mediated NP uptake by a desired cell type. We demonstrate that pre-coating silica NPs with gamma-globulins (γ-globulins) produced a protein corona that was enriched with opsonins, such as immunoglobulins. Although immunoglobulins are ligands that bind to receptors on macrophages and elicit phagocytois, the opsonin-rich protein corona did not increase NP uptake by macrophage RAW 264.7 cells. Immunolabeling experiments indicated that the binding of opsonins to their target cell surface receptors was impeded by other proteins in the corona. Thus, corona-mediated NP targeting strategies must optimize both the recruitment of the desired plasma proteins as well as their accessibility and orientation in the corona layer.

Keywords
Nanoparticle; Targeted delivery; Protein corona; Immunoglobulins; Fc receptor; Opsonins
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Impact of protein pre-coating on the protein corona composition and nanoparticle cellular uptake
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 75, January 2016, Pages 295–304
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us