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Bio-inspired configurable multiscale extracellular matrix-like structures for functional alignment and guided orientation of cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
5555 400 2015 7 PDF Available
Title
Bio-inspired configurable multiscale extracellular matrix-like structures for functional alignment and guided orientation of cells
Abstract

Inspired by the hierarchically organized protein fibers in extracellular matrix (ECM) as well as the physiological importance of multiscale topography, we developed a simple but robust method for the design and manipulation of precisely controllable multiscale hierarchical structures using capillary force lithography in combination with an original wrinkling technique. In this study, based on our proposed fabrication technology, we approached a conceptual platform that can mimic the hierarchically multiscale topographical and orientation cues of the ECM for controlling cell structure and function. We patterned the polyurethane acrylate-based nanotopography with various orientations on the microgrooves, which could provide multiscale topography signals of ECM to control single and multicellular morphology and orientation with precision. Using our platforms, we found that the structures and orientations of fibroblast cells were greatly influenced by the nanotopography, rather than the microtopography. We also proposed a new approach that enables the generation of native ECM having nanofibers in specific three-dimensional (3D) configurations by culturing fibroblast cells on the multiscale substrata. We suggest that our methodology could be used as efficient strategies for the design and manipulation of various functional platforms, including well-defined 3D tissue structures for advanced regenerative medicine applications.

Keywords
Multiscale structure; Extracellular matrix; Cell function; Scaffold; Tissue engineering
First Page Preview
Bio-inspired configurable multiscale extracellular matrix-like structures for functional alignment and guided orientation of cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 69, November 2015, Pages 158–164
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering