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Enabling non-invasive assessment of an engineered endothelium on ePTFE vascular grafts without increasing oxidative stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
5568 400 2015 11 PDF Available
Title
Enabling non-invasive assessment of an engineered endothelium on ePTFE vascular grafts without increasing oxidative stress
Abstract

Magnetic resonance imaging (MRI) in combination with contrast enhancement is a potentially powerful tool to non-invasively monitor cell distribution in tissue engineering and regenerative medicine. The most commonly used contrast agent for cell labeling is super paramagnetic iron oxide nanoparticles (SPIONs). However, uptake of SPIONs triggers the production of reactive oxygen species (ROS) in cells often leading to a pro-inflammatory phenotype. The objective of this study was to develop a labeling system to non-invasively visualize an engineered endothelium in vascular grafts without creating excessive oxidative stress. Specifically, we investigated: (1) chitosan-coated SPIONs (CSPIONs) as an antioxidant contrast agent for contrast enhancement, and (2) poly(1,8-octamethylene citrate) (POC) as an antioxidant interface to support cell adhesion and function of labeled cells on the vascular graft. While SPION-labeled endothelial cells (ECs) experienced elevated ROS formation and altered cell morphology, CSPION-labeled ECs cultured on POC-coated surfaces mitigated SPION-induced ROS formation and maintained EC morphology, phenotype, viability and functions. A monolayer of labeled ECs exhibited sufficient contrast with T2-weighed MR imaging. CSPION labeling of endothelial cells in combination with coating the graft wall with POC allows non-invasive monitoring of an engineered endothelium on ePTFE grafts without increasing oxidative stress.

Keywords
Super paramagnetic iron oxide nanoparticles (SPIONs); Endothelial cells (ECs); Reactive oxygen species (ROS); Chitosan; Poly (1,8-octanediol-co-citric acid) (POC); Magnetic resonance imaging (MRI)
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Enabling non-invasive assessment of an engineered endothelium on ePTFE vascular grafts without increasing oxidative stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 69, November 2015, Pages 110–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us