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Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation

Paper ID Volume ID Publish Year Pages File Format Full-Text
5579 401 2015 8 PDF Available
Title
Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation
Abstract

The alteration of cellular functions by anchoring macromolecules to specified organelles may reveal a new area of therapeutic potential and clinical treatment. In this work, a unique phenotype was evoked by influencing cellular behavior through the modification of subcellular structures with genetically targetable macromolecules. These fluorogen-functionalized polymers, prepared via controlled radical polymerization, were capable of exclusively decorating actin, cytoplasmic, or nuclear compartments of living cells expressing localized fluorgen-activating proteins. The macromolecular fluorogens were optimized by establishing critical polymer architecture-biophysical property relationships which impacted binding rates, binding affinities, and the level of internalization. Specific labeling of subcellular structures was realized at nanomolar concentrations of polymer, in the absence of membrane permeabilization or transduction domains, and fluorogen-modified polymers were found to bind to protein intact after delivery to the cytosol. Cellular motility was found to be dependent on binding of macromolecular fluorogens to actin structures causing rapid cellular ruffling without migration.

Keywords
Polymer modified cells; Targeting polymers to subcellular organelles; Genetically targetable macromolecules; Subcellular imaging; Protein polymer hybrids; Biomaterial structure-property relationships; Atom transfer radical polymerization (ATRP); Fluorogen
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Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 66, October 2015, Pages 1–8
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us