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Poly-l-arginine based materials as instructive substrates for fibroblast synthesis of collagen

Paper ID Volume ID Publish Year Pages File Format Full-Text
5605 406 2015 11 PDF Available
Title
Poly-l-arginine based materials as instructive substrates for fibroblast synthesis of collagen
Abstract

The interactions of cells and surrounding tissues with biomaterials used in tissue engineering, wound healing, and artificial organs ultimately determine their fate in vivo. We have demonstrated the ability to tune fibroblast responses with the use of varied material chemistries. In particular, we examined cell morphology, cytokine production, and collagen fiber deposition angles in response to a library of arginine-based polymeric materials. The data presented here shows a large range of vascular endothelial growth factor (VEGF) secretion (0.637 ng/106 cells/day to 3.25 ng/106 cells/day), cell migration (∼15 min < persistence time < 120 min, 0.11 μm/min < speed < 0.23 μm/min), and cell morphology (0.039 < form factor (FF) < 0.107). Collagen orientation, quantified by shape descriptor (D) values that ranges from 0 to 1, representing completely random (D = 0) to aligned (D = 1) fibers, exhibited large variation both in vitro and in vivo (0.167 < D < 0.36 and 0.17 < D < 0.52, respectively). These findings demonstrate the ability to exert a certain level of control over cellular responses with biomaterials and the potential to attain a desired cellular response such as, increased VEGF production or isotropic collagen deposition upon exposure to these materials in wound healing and tissue engineering applications.

Keywords
Fibroblast; Collagen; Collagen structure; Cell morphology
First Page Preview
Poly-l-arginine based materials as instructive substrates for fibroblast synthesis of collagen
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 63, September 2015, Pages 47–57
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering