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Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe

Paper ID Volume ID Publish Year Pages File Format Full-Text
5617 406 2015 9 PDF Available
Title
Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe
Abstract

Sulfur dioxide (SO2) and its derivatives sulfite and bisulfite play important roles in biological systems. However, in vivo detection of sulfite/bisulfite remains challenging. In this study, we developed a dinuclear Ir(III) complex (Ir4) as a two-photon phosphorescent probe for sulfite and bisulfite. Ir4 selectively and rapidly responded, with high sensitivity, to sulfite/bisulfite over other bio-related ions and molecules. One-photon and two-photon microscopy images revealed that Ir4 preferentially targeted mitochondria and was capable of imaging biological sulfite/bisulfite levels in vitro and in vivo. In situ sulfite generation in Caenorhabditis elegans was visualized by two-photon excitation real-time imaging. Finally, Ir4 was employed to monitor sulfite distribution in rat brain and other tissues. This study is the first report of the direct visualization of SO2 derivatives in vivo. These results provide new insights into the biological importance of SO2.

Keywords
Biological SO2; Iridium(III) complex; Two-photon probe; Caenorhabditis elegans; Mice tissues
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Direct imaging of biological sulfur dioxide derivatives in vivo using a two-photon phosphorescent probe
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 63, September 2015, Pages 128–136
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us