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In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus

Paper ID Volume ID Publish Year Pages File Format Full-Text
5624 407 2015 10 PDF Available
Title
In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus
Abstract

Nanomaterials conjugated with biomacromolecules, including viruses, have great potential for in vivo applications. Therefore, it is important to evaluate the safety of nanoparticle-conjugated macromolecule biomaterials (Nano-mbio). Although a number of studies have assessed the risks of nanoparticles and macromolecule biomaterials in living bodies, only a few of them investigated Nano-mbios. Here we evaluated the in vivo safety profile of a quantum dot-conjugated baculovirus (Bq), a promising new Nano-mbio, in mice. Each animal was injected twice intraperitoneally with 50 μg virus protein labelled with around 3*10-5nmol conjugated qds. Control animals were injected with PBS, quantum dots, baculovirus, or a mixture of quantum dots and baculovirus. Blood, tissues and body weight were analysed at a series of time points following both the first and the second injections. It turned out that the appearance and behaviour of the mice injected with Bq were similar to those injected with baculovirus alone. However, combination of baculovirus and quantum dot (conjugated or simply mixed) significantly induced stronger adaptive immune responses, and lead to a faster accumulation and longer existence of Cd in the kidneys. Thus, despite the fact that both quantum dot and baculovirus have been claimed to be safe in vivo, applications of Bq in vivo should be cautious. To our knowledge, this is the first study examining the interaction between a nanoparticle-conjugated virus and a living body from a safety perspective, providing a basis for in vivo application of other Nano-mbios.

Keywords
Quantum dot-conjugated baculovirus (Bq); Mice; Toxicity; Immune response; Kinetic characteristicBq, quantum dots conjugated baculovirus; qds, quantum dots; bv, baculovirus; Nano-mbio, nanoparticle conjugated macromolecular biomaterial; Nano-v, nanoparticl
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 64, September 2015, Pages 78–87
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us