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Site-selective in situ growth of fluorescent polymer–antibody conjugates with enhanced antigen detection by signal amplification

Paper ID Volume ID Publish Year Pages File Format Full-Text
5629 407 2015 8 PDF Available
Title
Site-selective in situ growth of fluorescent polymer–antibody conjugates with enhanced antigen detection by signal amplification
Abstract

This paper reports a new and general in situ methodology to grow fluorescent polymer conjugates from the interchain disulfide bridging sites of a monoclonal antibody. Atom transfer radical polymerization (ATRP) initiators were attached to a monoclonal antibody at its interchain disulfide bridging sites by disulfide re-bridging to yield a macroinitiator. Subsequent in situ ATRP of PEG-like monomers with dye-functionalized monomers from the macroinitiator formed antibody-polymer-dye conjugates with site-selectivity and tunable dye-to-antibody ratios. Notably, these conjugates can amplify antigen detection signal by reducing label-density dependent fluorescence quenching and by increasing dye-to-antibody ratios. The method developed may be applicable to a variety of antibodies, dyes and drugs to create a number of antibody-polymer-dye/drug conjugates for advanced diagnosis and therapy of diseases.

Keywords
Antibody conjugate; Polymer conjugate; Antigen detection; Signal amplification
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Site-selective in situ growth of fluorescent polymer–antibody conjugates with enhanced antigen detection by signal amplification
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 64, September 2015, Pages 2–9
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us