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Controlled surface topography regulates collective 3D migration by epithelial–mesenchymal composite embryonic tissues

Paper ID Volume ID Publish Year Pages File Format Full-Text
5638 410 2015 9 PDF Available
Title
Controlled surface topography regulates collective 3D migration by epithelial–mesenchymal composite embryonic tissues
Abstract

Cells in tissues encounter a range of physical cues as they migrate. Probing single cell and collective migratory responses to physically defined three-dimensional (3D) microenvironments and the factors that modulate those responses are critical to understanding how tissue migration is regulated during development, regeneration, and cancer. One key physical factor that regulates cell migration is topography. Most studies on surface topography and cell mechanics have been carried out with single migratory cells, yet little is known about the spreading and motility response of 3D complex multi-cellular tissues to topographical cues. Here, we examine the response to complex topographical cues of microsurgically isolated tissue explants composed of epithelial and mesenchymal cell layers from naturally 3D organized embryos of the aquatic frog Xenopus laevis. We control topography using fabricated micropost arrays (MPAs) and investigate the collective 3D migration of these multi-cellular systems in these MPAs. We find that the topography regulates both collective and individual cell migration and that dense MPAs reduce but do not eliminate tissue spreading. By modulating cell size through the cell cycle inhibitor Mitomycin C or the spacing of the MPAs we uncover how 3D topographical cues disrupt collective cell migration. We find surface topography can direct both single cell motility and tissue spreading, altering tissue-scale processes that enable efficient conversion of single cell motility into collective movement.

Keywords
Morphogenesis; Microstructure; Soft tissue biomechanics; Cell mechanics; Topology
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Controlled surface topography regulates collective 3D migration by epithelial–mesenchymal composite embryonic tissues
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 58, July 2015, Pages 1–9
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us