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In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules

Paper ID Volume ID Publish Year Pages File Format Full-Text
5688 417 2015 10 PDF Available
Title
In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules
Abstract

Glycation is one of the major processes responsible for skin aging through induction of the detrimental formation of advanced glycation end-products (AGEs). We developed an innovative tissue-engineered skin combining both a capillary-like and a nerve networks and designed a protocol to induce continuous AGEs formation by a treatment with glyoxal. We determined the optimal concentration of glyoxal to induce AGEs formation identified by carboxymethyl-lysin expression while keeping their toxic effects low. We showed that our tissue-engineered skin cultured for 44 days and treated with 200 μm glyoxal for 31 days displayed high carboxymethyl-lysine expression, which induced a progressively increased alteration of its capillary and nerve networks between 28 and 44 days. Moreover, it produced an epidermal differentiation defect evidenced by the lack of loricrin and filaggrin expression in the epidermis. These effects were almost completely prevented by addition of aminoguanidine 1.5 mm, an anti-glycation compound, and only slightly decreased by alagebrium 500 μm, an AGE-breaker molecule.This tissue-engineered skin model is the first one to combine a capillary and nerve network and to enable a continuous glycation over a long-term culture period. It is a unique tool to investigate the effects of glycation on skin and to screen new molecules that could prevent AGEs formation.

Keywords
Skin; Glyoxal; Carboxymethyl lysine; Advanced glycation end products; AngiogenesisAGE, advanced glycation end-products; CML, N-carboxymethyl-lysine; ECM, extracellular matrix
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In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 51, May 2015, Pages 216–225
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us