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Cold drawn bioabsorbable ferrous and ferrous composite wires: An evaluation of in vitro vascular cytocompatibility ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
569 51 2013 11 PDF Available
Title
Cold drawn bioabsorbable ferrous and ferrous composite wires: An evaluation of in vitro vascular cytocompatibility ☆
Abstract

A systematic approach is applied to quantify the impact of bioabsorbable metals on human vascular endothelial cells (EC) and aortic smooth muscle cells (SMC) with the aim of optimizing bioabsorbable endovascular stent development. Composite wires comprising novel combinations of Fe, Mn, Mg, and Zn were produced and fabricated into tubular mesh stents. The stents were incubated with primary EC in order to assess attachment and cell proliferation. Migration of SMCs from the vessel medial wall to the target lesion site following recanalization of an atherosclerotic artery is important in the process of neointimal hyperplasia. Metal ion species were assayed for their impact on cell migration and survival at concentrations ranging from 0.037 to 10 mM. An MTT-based assay was used to assess cytotoxicity after insult with various metal ion concentrations. Fe2+ and Fe3+ ion species were found to repress the migration of SMCs across a porous polycarbonate track etch membrane at concentrations of 1 mM. Mn2+ promoted SMC migration at a concentration of 1 mM, however, this effect was quenched when Fe2+ was included. Mg2+ was found to significantly increase SMC migration at concentrations above 1 mM. Cell survival was not reduced after 24 h insult with concentrations of Mg2+ up to 10 mM. LD50 concentrations of greater than 1 mM were found for Mg2+, Fe2+, Fe3+, and Fe2+ with 35 wt.% Mn2+. Significantly greater numbers of EC attached to bioabsorbable metal species compared with 316L stainless steel. Good EC coverage and proliferation were observed for all tested materials up to 120 h.

Graphical abstractA systematic approach is applied to quantify the impact of bioabsorbable metals on human vascular endothelial cells (EC) and aortic smooth muscle cells (SMC). Composite wires comprising novel combinations of Fe, Mn, Mg and Zn were produced and fabricated into tubular mesh stents. Stents were incubated with primary EC in order to assess attachment and cell proliferation. Metal ion species were assayed for impact on cell migration and survival at concentrations ranging from 0.037 to 10 mM. An MTT-based assay was used to assess cytotoxicity after insult with various metal ion concentrations.Fe2+ and Fe3+ ion species were found to repress the migration of SMCs at concentrations of 1 mM (A) across a porous polycarbonate-track-etch membrane. Mn2+ promoted SMC migration at a concentration of 1 mM (B), however, this effect was quenched when Fe2+ was included (C). Mg2+ was found to significantly increase SMC migration beyond a concentration of 1 mM. Cell survival was not reduced after 24-hour insult with concentrations of Mg2+ up to 10 mM (D). LD50 concentrations of greater than 1 mM were found for Mg2+, Fe2+, Fe3+, and Fe2+ with 35 w/w% Mn2+. Significantly greater numbers of EC attached to bioabsorbable metal species compared to 316L stainless steel (E). Good endothelial cell coverage and proliferation were observed for all tested materials out to 120 hours (F).Figure optionsDownload full-size imageDownload high-quality image (224 K)Download as PowerPoint slide

Keywords
Bioabsorbable stent; Biodegradable material; Endovascular stent; Metal cytotoxicity
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Cold drawn bioabsorbable ferrous and ferrous composite wires: An evaluation of in vitro vascular cytocompatibility ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 9, Issue 10, November 2013, Pages 8574–8584
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us