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Cell surface modification with ssDNA–PEG–lipid for analysing intercellular interactions between different cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
5713 419 2015 10 PDF Available
Title
Cell surface modification with ssDNA–PEG–lipid for analysing intercellular interactions between different cells
Abstract

Intercellular interactions are important in the development, immune responses, and functions of tissues and organs. Few methods are currently available for experimentally inducing and analysing cell–cell interaction in vitro. Here I propose a method to induce cell–cell attachment by cell surface modification with single-stranded DNA (ssDNA) and poly(ethylene glycol)-conjugated phospholipid (PEG–lipid) derivatives. The incorporation of an ssDNA pair (polyA20 and polyT20) into the cell membranes of two different cells was utilized to attach distinct cells through DNA hybridization. This technique enabled induction of cell–cell attachment between the same cell lines or different cell lines by controlling the contact area of two cells. Using this method, I investigated intercellular interactions, including the cell-in-cell invasion process, without impairing the interaction. I found that the normal cells MCF-10A were internalized into the cancer cells MCF-7, and that the intercellular interactions between them mainly involved the interaction of E-cadherin.

Keywords
Surface modification; Poly(ethylene glycol)-conjugated phospholipid (PEG–lipid); Cell–cell interaction; Cell–cell attachment; DNA
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Cell surface modification with ssDNA–PEG–lipid for analysing intercellular interactions between different cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 48, April 2015, Pages 119–128
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us