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Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels

Paper ID Volume ID Publish Year Pages File Format Full-Text
5730 423 2015 12 PDF Available
Title
Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels
Abstract

We have generated a bioinspired tunable system of hyaluronic acid (HyA)-based hydrogels for Matrix-Assisted Cell Transplantation (MACT). With this material, we have independently evaluated matrix parameters such as adhesion peptide density, mechanical properties, and growth factor sequestering capacity, to engineer an environment that imbues donor cells with a milieu that promotes survival and engraftment with host tissues after transplantation. Using a versatile population of Sca-1+/CD45− cardiac progenitor cells (CPCs), we demonstrated that the addition of heparin in the HyA hydrogels was necessary to coordinate the presentation of TGFβ1 and to support the trophic functions of the CPCs via endothelial cell differentiation and vascular like tubular network formation. Presentation of exogenous TGFβ1 by binding with heparin improved differentiated CPC function by sequestering additional endogenously-produced angiogenic factors. Finally, we demonstrated that TGFβ1 and heparin-containing HyA hydrogels can promote CPC survival when implanted subcutaneously into murine hind-limbs and encouraged their participation in the ensuing neovascular response, which included blood vessels that had anastomosed with the host's blood vessels.

Keywords
Stem cell transplantation; Hyaluronic acid hydrogel; Growth factor sequestration; Neovascularization; Heparin; TGFβ1
First Page Preview
Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 47, April 2015, Pages 1–12
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering