fulltext.study @t Gmail

Fabrication of new single cell chip to monitor intracellular and extracellular redox state based on spectroelectrochemical method

Paper ID Volume ID Publish Year Pages File Format Full-Text
5758 426 2015 8 PDF Available
Title
Fabrication of new single cell chip to monitor intracellular and extracellular redox state based on spectroelectrochemical method
Abstract

Probing the local environment of target cells has been considered a challenging task due to the complexity of living cells. Here, we developed new single cell-based chip to investigate the intracellular and extracellular redox state of PC12 cells using spectroelectrochemical tool that combined surface-enhanced Raman scattering (SERS) and linear sweep voltammetry (LSV) techniques. PC12 cells immobilized on gold nanodots/ITO surface were subjected to LSV and their intracellular biochemical changes were successfully monitored by SERS simultaneously. Moreover, paired gold microelectrodes with micrometer-sized gap containing hexagonal array of gold nanodots were fabricated to detect electrochemical activity and changes in the redox environment of single PC12 cell based on SERS–LSV tool. This showed very effective detecting method. The used technology included the utilization of gold nanodots array inside micro-gap to enhance the Raman signals and the electrochemical activity of single cell. This could be used as an effective research tool to analyze cellular processes.

Keywords
Intracellular and extracellular redox state; Spectroelectrochemical; Single neural cell-chip; Gold microelectrodes; Gold nanodots array
First Page Preview
Fabrication of new single cell chip to monitor intracellular and extracellular redox state based on spectroelectrochemical method
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 40, February 2015, Pages 80–87
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us