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Intracellular accumulation and immunological properties of fluorescent gold nanoclusters in human dendritic cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
5768 428 2015 12 PDF Available
Title
Intracellular accumulation and immunological properties of fluorescent gold nanoclusters in human dendritic cells
Abstract

We have studied the effect of highly fluorescent gold nanoclusters (Au NCs) (∅ < 3 nm) stabilized by different ligands on the intracellular accumulation and immune response of human derived-monocyte dendritic cells (DCs). Results indicate that the high uptake efficiency of Au NCs is strongly related to their small size and to the nature of the ligand, with zwitterionic ligands being more effective than PEGylated ones. Evidence from flow cytometry and microscopy demonstrate time and concentration-dependent Au NCs internalization by endocytic pathway(s) involving amorphous and laminar organelles, while maintaining their discrete size and photoluminescence properties. The uptake of zwitterionic ligand-stabilized Au NCs induced very low cytotoxicity and a strong immunosuppressive response (Th1/Treg pattern), associated with a DC maturation state. This behavior contrasts to the effect of bigger particles (∼12 nm size) which induced a cytotoxic response involving Natural Killer (CD56) cells. Overall, this study stresses the critical importance of particle size and ligand type on the immunostimulation of DCs and highlights the remarkable potential of this new class of nanomaterial as a novel vaccine platform.

Keywords
Gold nanoclusters; Fluorescence; Cellular uptake; Immune response; Monocyte-derived dendritic cells
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Intracellular accumulation and immunological properties of fluorescent gold nanoclusters in human dendritic cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 43, March 2015, Pages 1–12
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us