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Synthetic β-sheet forming peptide amphiphiles for treatment of fungal keratitis

Paper ID Volume ID Publish Year Pages File Format Full-Text
5770 428 2015 6 PDF Available
Title
Synthetic β-sheet forming peptide amphiphiles for treatment of fungal keratitis
Abstract

Fungal keratitis is a leading cause of ocular morbidity. It is frequently misdiagnosed as bacterial keratitis, causing a delay in proper treatment. Furthermore, due to the lack of safe and effective anti-fungal agents for clinical use, treatment of fugal keratitis remains a challenge. In recent years, antimicrobial peptides (AMPs) have received considerable attention as potent and broad-spectrum antimicrobial agents with the potential to overcome antibiotics resistance. We previously reported the design of short synthetic β-sheet forming peptides (IKIK)2–NH2 and (IRIK)2–NH2 with excellent antimicrobial activities and selectivities against various clinically relevant microorganisms, including Gram-positive Staphylococcus epidermidis and Staphylococcus aureus, Gram-negative Escherichia coli and Pseudomonas aeruginosa, and yeast Candida albicans (C. albicans). In this study, we evaluated the application of the two most promising synthetic β-sheet forming peptide candidates for in vivo fungal keratitis treatment in comparison with the commercially available amphotericin B. It was found that topical solutions of the designed peptides are safe, and as effective as the clinically used amphotericin B. Compared to the costly and unstable amphotericin B, (IKIK)2–NH2 and (IRIK)2–NH2 are water-soluble, less expensive and stable. Thus, the synthetic β-sheet forming peptides are presented as promising candidates for the treatment of fungal keratitis.

Keywords
Fungal keratitis; Antibiotics resistance; β-sheet forming peptide; Antifungal; Anti-biofilm
First Page Preview
Synthetic β-sheet forming peptide amphiphiles for treatment of fungal keratitis
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 43, March 2015, Pages 44–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering