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Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials

Paper ID Volume ID Publish Year Pages File Format Full-Text
5773 430 2014 13 PDF Available
Title
Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials
Abstract

The access to defined protein-based material systems is a major challenge in bionanotechnology and regenerative medicine. Exact control over sequence composition and modification is an important requirement for the intentional design of structure and function. Herein structural- and matrix proteins provide a great potential, but their large repetitive sequences pose a major challenge in their assembly. Here we introduce an integrative “one-vector-toolbox-platform” (OVTP) approach which is fast, efficient and reliable. The OVTP allows for the assembly, multimerization, intentional arrangement and direct translation of defined molecular DNA-tecton libraries, in combination with the selective functionalization of the yielded protein-tecton libraries. The diversity of the generated tectons ranges from elastine-, resilin, silk- to epitope sequence elements. OVTP comprises the expandability of modular biohybrid-materials via the assembly of defined multi-block domain genes and genetically encoded unnatural amino acids (UAA) for site-selective chemical modification. Thus, allowing for the modular combination of the protein-tecton library components and their functional expansion with chemical libraries via UAA functional groups with bioorthogonal reactivity. OVTP enables access to multitudes of defined protein-based biohybrid-materials for self-assembled superstructures such as nanoreactors and nanobiomaterials, e.g. for approaches in biotechnology and individualized regenerative medicine.

Keywords
Biohybrid-materials; ECM (extracellular matrix); Elastin-like-protein; Genetic engineering; Molecular tecton-libraries; Self-assembly2D, two-dimensional; 3D, three-dimensional; AA, amino acid; CL, cyclic-ligation; EbM, elastin based materials; ELP, elasti
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Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid-materials
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 31, October 2014, Pages 8767–8779
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us