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Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
5775 430 2014 10 PDF Available
Title
Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix
Abstract

Despite dramatic progress in infertility treatments and assisted reproduction, no effective therapies exist for complete loss of uterine structure and/or function. For such patients, genetic motherhood is possible only through gestational surrogacy or uterine transplantation. However, many ethical, social, technical and safety challenges accompany such approaches. A theoretical alternative is to generate a bioartificial uterus, which requires engineering of uterine architecture and appropriate cellular constituents. Here, rat uteri decellularization by aortic perfusion with detergents produced an underlying extracellular matrix together with an acellular, perfusable vascular architecture. Uterine-like tissues were then regenerated and maintained in vitro for up to 10 d through decellularized uterine matrix (DUM) reseeding with adult and neonatal rat uterine cells and rat mesenchymal stem cells followed by aortic perfusion in a bioreactor. Furthermore, DUM placement onto a partially excised uterus yielded recellularization and regeneration of uterine tissues and achievement of pregnancy nearly comparable to the intact uterus. These results suggest that DUM could be used for uterine regeneration, and provides insights into treatments for uterine factor infertility.

Keywords
Cell culture; ECM (extracellular matrix); Organ culture; Scaffold
First Page Preview
Partial regeneration and reconstruction of the rat uterus through recellularization of a decellularized uterine matrix
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 31, October 2014, Pages 8791–8800
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering