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Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation

Paper ID Volume ID Publish Year Pages File Format Full-Text
5792 431 2015 11 PDF Available
Title
Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation
Abstract

Understanding protein adsorption and resultant conformation changes on modified and unmodified silicon dioxide surfaces is a subject of keen interest in biosensors, microfluidic systems and for medical diagnostics. However, it has been proven difficult to investigate the kinetics of the adsorption process on these surfaces as well as understand the topic of the denaturation of proteins and its effect on enzyme activity. A highly sensitive optical whispering gallery mode (WGM) resonator was used to study a catalytic enzyme's adsorption processes on different silane modified glass substrates (plain glass control, DETA, 13F, and SiPEG). The WGM sensor was able to obtain high resolution kinetic data of glucose oxidase (GO) adsorption with sensitivity of adsorption better than that possible with SPR. The kinetic data, in combination with a functional assay of the enzyme activity, was used to test hypotheses on adsorption mechanisms. By fitting numerical models to the WGM sensograms for protein adsorption, and by confirming numerical predictions of enzyme activity in a separate assay, we were able to identify mechanisms for GO adsorption on different alkylsilanes and infer information about the adsorption of protein on nanostructured surfaces.

Keywords
Biosensor; Modeling; Protein adsorption; Surface modification; Nanobiotechnology; Self-assembled monolayer
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Combining an optical resonance biosensor with enzyme activity kinetics to understand protein adsorption and denaturation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 38, January 2015, Pages 86–96
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Any Questions? feel free to contact us