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Collagen mimetic peptide engineered M13 bacteriophage for collagen targeting and imaging in cancer

Paper ID Volume ID Publish Year Pages File Format Full-Text
5820 437 2014 10 PDF Available
Title
Collagen mimetic peptide engineered M13 bacteriophage for collagen targeting and imaging in cancer
Abstract

Collagens are over-expressed in various human cancers and subsequently degraded and denatured by proteolytic enzymes, thus making them a target for diagnostics and therapeutics. Genetically engineered bacteriophage (phage) is a promising candidate for the development of imaging or therapeutic materials for cancer collagen targeting due to its promising structural features. We genetically engineered M13 phages with two functional peptides, collagen mimetic peptide and streptavidin binding peptide, on their minor and major coat proteins, respectively. The resulting engineered phage functions as a therapeutic or imaging material to target degraded and denatured collagens in cancerous tissues. We demonstrated that the engineered phages are able to target and label abnormal collagens expressed on A549 human lung adenocarcinoma cells after the conjugation with streptavidin-linked fluorescent agents. Our engineered collagen binding phage could be a useful platform for abnormal collagen imaging and drug delivery in various collagen-related diseases.

Keywords
Collagen; Bacteriophage; Cancer; Targeting; Imaging
First Page Preview
Collagen mimetic peptide engineered M13 bacteriophage for collagen targeting and imaging in cancer
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 33, November 2014, Pages 9236–9245
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering