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Cell adhesion mechanisms on laterally mobile polymer films

Paper ID Volume ID Publish Year Pages File Format Full-Text
5831 438 2014 8 PDF Available
Title
Cell adhesion mechanisms on laterally mobile polymer films
Abstract

In contrast with the majority of substrates used to study cell adhesion, the natural extracellular matrix (ECM) is dynamic and remodeled over time. Here we use amphiphilic block copolymers to create self-assembled supported films with tunable lateral mobility. These films are intended to serve as partial mimics of the ECM in order to better understand cell adhesion responses, specifically in the context of dynamic substrates. Block copolymers are end-labeled with RGD peptide ligands to allow for integrin-mediated cell adhesion, and the addition of a trace hydrophobic homopolymer is used to control the film lateral mobility. We find that NIH 3T3 fibroblasts cultured on these biomimetic films exhibit non-linear spreading behavior in response to substrate mobility. In the absence of RGD ligands, however, fibroblasts do not spread. Employing quantitative analysis of focal adhesions (FA) and integrin ligation, we discover the presence of FA-dependent and FA-independent mechanisms responsible for the biphasic cell spreading behavior. The use of designed biomimetic platforms therefore yields insight into ECM mechanosensing by revealing that cells can engage distinct mechanisms to promote adhesion onto substrates with different time-dependent properties.

Keywords
Block copolymers; Self-assembly; Bilayers; Lateral mobility; Focal adhesions
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Cell adhesion mechanisms on laterally mobile polymer films
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 17, June 2014, Pages 4827–4834
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us