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The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
5846 441 2014 11 PDF Available
Title
The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis
Abstract

Several chemokines are important in muscle myogenesis and in the recruitment of muscle precursors during muscle regeneration. Among these, the SDF-1α chemokine (CXCL12) is a potent chemoattractant known to be involved in muscle repair. SDF-1α was loaded in polyelectrolyte multilayer films made of poly(l-lysine) and hyaluronan to be delivered locally to myoblast cells in a matrix-bound manner. The adsorbed amounts of SDF-1α were tuned over a large range from 100 ng/cm2 to 5 μg/cm2, depending on the initial concentration of SDF-1α in solution, its pH, and on the film crosslinking extent. Matrix-bound SDF-1α induced a striking increase in myoblast spreading, which was revealed when it was delivered from weakly crosslinked films. It also significantly enhanced cell migration in a dose-dependent manner, which again depended on its presentation by the biopolymeric film. The low-crosslinked film was the most efficient in boosting cell migration. Furthermore, matrix-bound SDF-1α also increased the expression of myogenic markers but the fusion index decreased in a dose-dependent manner with the adsorbed amount of SDF-1α. At high adsorbed amounts of SDF-1α, a large number of Troponin T-positive cells had only one nucleus. Overall, this work reveals the importance of the presentation mode of SDF-1α to emphasize its effect on myogenic processes. These films may be further used to provide insight into the role of SDF-1α presented by a biomaterial in physiological or pathological processes.

Keywords
Polyelectrolyte multilayer film; Drug delivery; SDF-1α/CXCL12; CXCR4; Myoblast; MigrationBMP, bone morphogenetic protein; EDC, 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide; HA, hyaluronan; PDMS, polydimethylsiloxane; PEM, polyelectrolyte multilayer fil
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The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 15, May 2014, Pages 4525–4535
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us