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Mechanistic insights into response of Staphylococcus aureus to bioelectric effect on polypyrrole/chitosan film

Paper ID Volume ID Publish Year Pages File Format Full-Text
5855 444 2014 9 PDF Available
Title
Mechanistic insights into response of Staphylococcus aureus to bioelectric effect on polypyrrole/chitosan film
Abstract

Treatment of biofilm-related infections in orthopedics remains a serious clinical challenge. It is known that an electric current can significantly enhance the potency of some antibiotics against biofilms (bioelectric effect) but the uncertainty of the mechanisms and the electrolytic cell-like system used in previous studies limit its applications. Herein, the behavior of Staphylococcus aureus (S. aureus) on an electrically conductive polypyrrole/chitosan film upon passage of a direct current (DC) through the film was investigated in the absence and presence of gentamicin. The killing efficacy of the bacteria within the biofilm by gentamicin was greatly enhanced by the DC treatment. From an analysis of the gene expression by the biofilm bacteria after treatment with gentamicin, DC and their combination, it is postulated that the promotion of bacterial autolysis by DC treatment is responsible for the enhanced susceptibility of biofilm S. aureus to gentamicin. This postulate is supported by an increase in the amount of extracellular deoxyribonucleic acid and adenosine triphosphate, and the appearance of disrupted bacterial cells in the biofilm after DC treatment. These findings provide a new insight into the interaction between DC and bacteria, and offer potential benefits for the treatment of infections in orthopedics.

Keywords
Autolysis; Bioelectric effect; Biofilm; Direct current; Gentamicin; Staphylococcus aureus
First Page Preview
Mechanistic insights into response of Staphylococcus aureus to bioelectric effect on polypyrrole/chitosan film
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 27, September 2014, Pages 7690–7698
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering