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Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance

Paper ID Volume ID Publish Year Pages File Format Full-Text
5860 444 2014 12 PDF Available
Title
Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance
Abstract

The topography of the extracellular microenvironment influences cell morphology, provides conduct guidance and directs cell differentiation. Aspect ratio and dimension of topography have been shown to affect cell behaviours, but the ability and mechanism of depth-sensing is not clearly understood. We showed that murine neural progenitor cells (mNPCs) can sense the depth of the micro-gratings. Neurite elongation, alignment and neuronal differentiation were observed to increase with grating depth. We proposed a mechanism for depth-sensing by growing neurites: filopodial adhesion in the growth cones favour elongation but the bending rigidity of the neurite cytoskeleton resists it. Thus, perpendicular extension on deeper grooves is unfavourable as neurites need to bend over a larger angle. A quantitative model was developed and its prediction of neurite growth on gratings fit well with the experimental data. The results indicated that mNPC fate can be directed by appropriately designed patterned surfaces.

Keywords
Neural progenitor cells; Nanotopography; Neural tissue engineering; Modelling; Neuronal differentiation
First Page Preview
Extending neurites sense the depth of the underlying topography during neuronal differentiation and contact guidance
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 27, September 2014, Pages 7750–7761
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering