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A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites

Paper ID Volume ID Publish Year Pages File Format Full-Text
5908 445 2014 12 PDF Available
Title
A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites
Abstract

Gene therapy provides a new hope for previously “incurable” diseases. Low gene transfection efficiency, however, is the bottle-neck to the success of gene therapy. It is very challenging to develop non-viral nanocarriers to achieve ultra-high gene transfection efficiencies. Herein, we report a novel design of “tight binding-but-detachable” lipid-nanoparticle composite to achieve ultrahigh gene transfection efficiencies of 60∼82%, approaching the best value (∼90%) obtained using viral vectors. We show that Fe@CNPs nanoparticles coated with LP-2000 lipid molecules can be used as gene carriers to achieve ultra-high (60–80%) gene transfection efficiencies in HeLa, U-87MG, and TRAMP-C1 cells. In contrast, Fe@CNPs having surface-covalently bound N,N,N-trimethyl-N-2-methacryloxyethyl ammonium chloride (TMAEA) oligomers can only achieve low (23–28%) gene transfection efficiencies. Similarly ultrahigh gene transfection/expression was also observed in zebrafish model using lipid-coated Fe@CNPs as gene carriers. Evidences for tight binding and detachability of DNA from lipid-nanoparticle nanocarriers will be presented.

Keywords
Gene transfection; Gene therapy; Nanocarriers; Zebrafish; Cytotoxicity; Carbon nanoparticles
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A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 28, September 2014, Pages 8261–8272
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us