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Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
5925 448 2014 10 PDF Available
Title
Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes
Abstract

The purification of human adipose-derived stem cells (hADSCs) from human adipose tissue cells (stromal vascular fraction) was investigated using membrane filtration through poly(lactide-co-glycolic acid)/silk screen hybrid membranes. Membrane filtration methods are attractive in regenerative medicine because they reduce the time required to purify hADSCs (i.e., less than 30 min) compared with conventional culture methods, which require 5–12 days. hADSCs expressing the mesenchymal stem cell markers CD44, CD73, and CD90 were concentrated in the permeation solution from the hybrid membranes. Expression of the surface markers CD44, CD73, and CD99 on the cells in the permeation solution from the hybrid membranes, which were obtained using 18 mL of feed solution containing 50 × 104 cells, was statistically significantly higher than that of the primary adipose tissue cells, indicating that the hADSCs can be purified in the permeation solution by the membrane filtration method. Cells expressing the stem cell-associated marker CD34 could be successfully isolated in the permeation solution, whereas CD34+ cells could not be purified by the conventional culture method. The hADSCs in the permeation solution demonstrated a superior capacity for osteogenic differentiation based on their alkali phosphatase activity, their osterix gene expression, and the results of mineralization analysis by Alizarin Red S and von Kossa staining compared with the cells from the suspension of human adipose tissue. These results suggest that the hADSCs capable of osteogenic differentiation preferentially permeate through the hybrid membranes.

Keywords
Adipose-derived stem cells; Bioseparation; Poly(lactide-co-glycolic acid); Differentiation; Osteoblast; Membrane purification
First Page Preview
Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 14, May 2014, Pages 4278–4287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering